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Karo honey tek

Karo honey tek

This tek is a method for multiplying spore syringes by growing in liquid, specifically water mixed with honey. The tek is flexible in that it can easily be done in different size jars as long as the proper ratio is maintained between the sugars and the water. See: Karo Tek. For those who figure that close is good enough, roughly 1 tablespoon of honey per pint will work. The basic idea then is to get a jar with a good lid, canning jars like commonly used in pf tek work great.

The lid can be drilled and the hole covered with a filter disk or stuffed with polyfil to allow spores to be injected after the mix has been sterilized. Alternatively, one can use a glovebox and simply crack the lid a little to inoculate. After the lid has been prepared, add the water and honey and stir until the honey dissolves. It's easier if warm water is used. Then tighten the lid and pressure cook or steam for 20 minutes. Don't overdo it because too much heat too long will ruin the mix by carmelizing the sugars.

After it cools to room temperature it is ready to be inoculated. Inject 1 or 2 cc's of spore solution into each jar. Alternatively, instead of spores, mycelial tissue from any part of any living shroom can be substituted.

Keep the inoculated jar warm in a clean environment. Within several days, strands of mycelial tissue will appears and begin to grow. Allow it to grow at least several days then it is ready to use. Try to get as much tissues as possible in the syringes, a nice thick mix will grow out very fast when used to inoculate jars, etc. Store excess in clean refrigerator or discard within a few more days as the mix is best used fresh.

In the fridge, it can last until the sugar runs out, about a month or so, usually. Honey Tek polish transl. See: Karo Tek For those who figure that close is good enough, roughly 1 tablespoon of honey per pint will work.Liquid Culture Basics. Liquid tissue cultures are used to expand mycelium into a liquid solution to inoculate your chosen substrate.

Like a multi-spore syringe, except the spores have germinated into a network. Since the spores are already germinated; colonization times are substantially faster and inoculated substrates have an edge over contamination with speed. In addition to the significant speed boost over MS multi spore inoculation, mycelium is not harmed by hydrogen peroxide H 2 O 2so this can be added to your substrate to help reduce contamination.

Normally you can not do this until the mycelium has started growing, because H 2 O 2 kills the spores.

One spore syringe can be made into gallons of liquid culture. One spore print, agar culture and mushroom tissue can also make gallons.

Also if the liquid inoculant is a clone generated from sectored agar or mushroom tissuethen each jar should show similar growth speeds and maturity. Medium Some nutrient sources are: Karo You want the clear one that has the red label; "Light with Vanilla".

DO NOT get the dark Karo, such as the one containing brown sugar Honey Non-organic has been known to work, but organic honey is highly recommended. Corn sugar Light malt extract or extra light. The lighter the better, because it will make it easier to observe growth in the jar. Dextrose glucose While only the above are recommended, because they have been tried and tested, other sources have been successfully used, such as organic maple syrup.

Household sugar sucrose shouldn't be used. Dextrose alone is usually not recommended, but will work, due to the lack of additional nutrients its growth may be slower but due to its clarity it may be easier to spot contamination.

Light malt extract and honey can be used alone. Additional nutrients can be added such as peptone, various flours could be used but it is much harder to determine the stage of mycelium growth due to cloudiness. Malt and Dextrose One member uses -- 1 tablespoon dry malt extract or dextrose to ml 1 cup water. Nanook from Nan's Nook uses -- 1 level teaspoon dextrose or light malt extract to 75ml water. A syringe without the needle inserted is good to use as a measuring device. It'll still put out viable mycelium in most situations.

karo honey tek

Mixing Once you have picked your method which ever suits you best or is easiest to get then its time to do some mixing. Optionally, water can be hot or warm before adding sugars to allow for quicker dissolving. Wrap top with aluminum foil and place jar in pressure cooker and slowly bring it up to 15 psi. Allow pressure cooker to cool before removing. Vacu-tainers You can bore a small hole big enough for a syringe needle in the top of a jar. Half pints work best for this Now put a blob of silicone sealant on it preferable transparent on both sides.

Swirl it around to make sure it is a centimeter thick around the hole on each side. This is a self healing inoculation point so you can add spores and suck up inoculant without having to open the jar after sterilization.

karo honey tek

If you band the jar tight before pressure cooking, it will form a vacuum and suck in spores, so you must only prick the injection spot quickly. If you leave the band loose, you should tighten it right after the pressure cooker has cooled down, as it will not have a vacuum seal.

You should always wipe your silicone injection spot and needle flame sterilize before with alcohol before inoculation. Agitation Some people add a piece of broken glass, a glass marble or a pebble to the jar before sterilization. Agitating allows you to cut up the mycellium which can form into an unsuckable clump in the jar. This is why wide 18 gauge or lower needles are preferable. A slightly more advanced method is adding a stir rod or just a 1" piece of non-insulated wire to the jar and using a magnetic stirring plate to agitate the mycelium.Nan's Nook : Archives : Liquid Culture.

For those who figure that close is good enough, roughly 1 tablespoon of honey per pint will work. The basic idea then is to get a jar with a good lid, canning jars like commonly used in pf tek work great.

The lid can be drilled and the hole covered with a filter disk or stuffed with polyfil to allow spores to be injected after the mix has been sterilized. Alternatively, one can use a glovebox and simply crack the lid a little to inoculate. After the lid has been preparedadd the water and honey and stir until the honey disolves. It's easier if warm water is used. Then tighten the lid and pressure cook or steam for 20 minutes.

Don't overdo it because too much heat too long will ruin the mix by carmelizing the sugars. After it cools to room temperature it is ready to be inoculated. Inject 1 or 2 cc's of spore solution into each jar. Alternatively, instead of spores, mycellial tissue from any part of any living shroom can be substituted.

Keep the inoculated jar warm in a clean environment. Within several days, strands of mycellial tissue will appears and begin to grow. Allow it to grow at least several days then it is ready to use. Try to get as much tissues as possible in the syringes, a nice thick mix will grow out very fast when used to inoculate jars, etc. Store excess in clean refridgerator or discard within a few more days as the mix is best used fresh.

In the 'fridge, it can last until the sugar runs out, about a month or so, usually.Log in or Sign up. I have a soft spot for agaricus and so I am thinking of keeping a liquid culture so that I can inoculate grain jars with it. This is all new for me so I have some questions for you keener growers How many times can a LC be transferred? How do I start a clean LC?

karo honey tek

How long can a LC be stored in the fridge? What is the best receipt for an agaricus LC? Lol, winner gets NichromeMar 1, I have read about honey cultures. Could it be tha simple and really work???

NichromeMar 2, Majik likes this. Joined: Jan 31, Messages: Best way would be to start from clean agar. I've started from spores, LC and agar and had issues eventually with all of them. LC certainly is easy, esp for allowing open-air inoculation through a SHIP, but I am not sure I trust it all that much based on mixed results. I have used the Karo Tek and the Honey Tek. Honey leaves weird proteins in the jar, but it seemed to colonize faster. Karo is nice and clear until myc starts growing, but you can caramelize it if you cook it too long.

People state that using slightly less is better than using slightly more. LC can be stored in the fridge for a few months. OtherOneMar 2, MajikMar 2, Before or after opening, Karo syrup may be stored at room temperature.

Storage conditions affect product quality.

Liquid Cultures

Light corn syrup may turn slightly yellow with age, but this is normal and not harmful. Karo syrup is safe to eat for an indefinite period of time regardless of whether it has been opened.

How to tell a good jar of mycelium from a bad or contaminated jar in liquid cultures

However, for best results we recommend using before the "Best by" date stamped on the container. Does corn syrup serve any function in recipes besides sweetening?

Corn syrup serves different functions in different types of recipes and in products you purchase. It controls sugar crystallization in candy, prevents the formation of ice crystals in frozen desserts, enhances fresh fruit flavor in jams and preserves, sweetens and thickens relishes. Corn syrup balances sweet and sour flavor profiles, and is therefore a key ingredient in many Asian dishes. When brushed onto baked ham, barbecued meats, baked vegetables or fresh fruit, it is an ideal glaze.

In baked goods, corn syrup holds moisture and maintains freshness longer. Karo light and dark corn syrups perform similarly in recipes and can usually be used interchangeably. Recipes usually specify which type to use but the choice may be guided by personal preference. Typically, light corn syrup is used when a delicately sweet flavor is desired, such as in fruit sauces and jams.

Karo light corn syrup is made with real vanilla.

Best newb way to create liquid mycelium cultures for reusing syringes (and saving $$)

Dark corn syrup is made with refiners' syrup, a type of molasses. With its more robust flavor and color, it is ideal for many baked goods. Both Karo light and dark corn syrups, along with Karo pancake syrup, are ideal toppings for pancakes, waffles, French toast, and biscuits. Karo Lite reduced calorie syrup can be substituted in many recipes calling for light or dark corn syrup, but is not recommended for cereal bars or candy making.

Can corn syrup be substituted for brown sugar or granulated sugar in recipes? Although corn syrup and granulated sugars are both sweeteners, it is not possible to use them interchangeably in recipes. Because corn syrup is a liquid, it cannot be substituted for granulated sugar without adjusting other ingredients, particularly in baked goods. For best results, follow recipes developed especially for corn syrup.

In sugar-sweetened beverages, however, it's easy to experiment with corn syrup as a ready-blending substitute.Log in or Sign up. ToniJul 31, The standard recipe is 0. So for ml of water you would use 0. Professor PinHeadJul 31, As always thank you very much Prof. Not a problem brother. Distilled water isn't necessary for liquid culturing mediums.

Tap water works great, becomes sterile during the PC run. Culturing medium additives, such as peptone or nutritional yeast, can be added to liquid culturing mediums at. I did not know there were so many possibilities, thanks for share that info.

karo honey tek

Have you tried apple juice at some time? ToniAug 1, Joined: Jul 20, Messages: If you have some colonized grain sitting around I personally would go with a grain lc. Shake a jar of grain, shoot sterile water into it, then suck the water back up into the syringe. Blood MeridianAug 2, ATM I only have one dish colonizing agar, but it is another possibility.

And after that can you use that grain for spawn? ToniAug 2, I've never had any issues with using it as spawn. Thanks guys, will have to try it. You must log in or sign up to reply here.Liquid tissue cultures are used to expand mycelium into a liquid solution to inoculate your chosen substrate. Like a multi-spore syringe, except the spores have germinated into a network. Since the spores are already germinated; colonization times are substantially faster and inoculated substrates have an edge over contamination with speed.

In addition to the significant speed boost over MS multi spore inoculation, mycelium is not harmed by hydrogen peroxide H 2 O 2so this can be added to your substrate to help reduce contamination. Normally you can not do this until the mycelium has started growing, because H 2 O 2 kills the spores. One spore syringe can be made into gallons of liquid culture.

One spore print, agar culture and mushroom tissue can also make gallons. Also if the liquid inoculant is a clone generated from sectored agar or mushroom tissuethen each jar should show similar growth speeds and maturity.

Household sugar sucrose shouldn't be used. Dextrose alone is usually not recommended, but will work, due to the lack of additional nutrients its growth may be slower but due to its clarity it may be easier to spot contamination. Light malt extract and honey can be used alone. Additional nutrients can be added such as peptone, various flours could be used but it is much harder to determine the stage of mycelium growth due to cloudiness.

These are the weights. Nanook from Nan's Nook uses - 1 level teaspoon dextrose or light malt extract to 75ml water. A syringe without the needle inserted is good to use as a measuring device.

It'll still put out viable mycelium in most situations. Once you have picked your method which ever suits you best or is easiest to get then its time to do some mixing. Optionally, water can be hot or warm before adding sugars to allow for quicker dissolving.

Wrap top with aluminum foil and place jar in pressure cooker and slowly bring it up to 15 psi. Allow pressure cooker to cool before removing. You can bore a small hole big enough for a syringe needle in the top of a jar.

Half pints work best for this Now put a blob of silicone sealant on it preferable transparent on both sides. Swirl it around to make sure it is a centimeter thick around the hole on each side.

This is a self healing inoculation point so you can add spores and suck up inoculant without having to open the jar after sterilization. If you band the jar tight before pressure cooking, it will form a vacuum and suck in spores, so you must only prick the injection spot quickly. If you leave the band loose, you should tighten it right after the pressure cooker has cooled down, as it will not have a vacuum seal. You should always wipe your silicone injection spot and needle flame sterilize before with alcohol before inoculation.

Some people add a piece of broken glass, a glass marble or a pebble to the jar before sterilization. Agitating allows you to cut up the mycellium which can form into an unsuckable clump in the jar. This is why wide 18 gauge or lower needles are preferable.

A slightly more advanced method is adding a stir rod or just a 1" piece of non-insulated wire to the jar and using a magnetic stirring plate to agitate the mycelium. This is the preferred method of agitation because it doesn't have the potential to get your lid filter polyfil for example wet when you shake the jar, which can lead to contamination.


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